Where repeat crosses gavehomogeneous counts and the
19 05 2009The figure is not to scale. 2002. These data show thattechnical variations, sampling error, and other random factorshave little effect on the recombination frequencies measuredin this study. Crossovers are not randomly distributed along chromosomes buttend to be clustered LICHTEN and GOLDMAN 1995 BAUDAT and NICOLAS1997 JEFFREYS et al. PF is the frequency of His Ad progeny is given as fraction of the total number of viable spores. These data suggest theexistence of more than one gene, each with small effect onrecombination, and that the parents of the and K874 strainsdescribed above carried different alleles of these genes.
Although there are multiple differences betweenthe cog region YEADON and CATCHESIDE 1999. Alleles in closeproximity experience coconversion more often than widely separatedsites HILLIKER et al. Recombination assay data are available in TablesA1 and A2 in an electronic appendix at contrast, recombination frequencies from crosses of strainswith the same cog, his3, and rec2 alleles can be variable. Since recombination frequencies do not vary between repeatsof the same cross, other factors must be affecting recombinationin these crosses. It is possible thereforethat recombination is initiated as much as times more frequentlyat cog than at cog.
Lys Ad frequencies were obtained from cog homozygotes A T12010 T4395, T12011 T4395, T12012 T4395, T12013 T4395, T12010 T11126, T12010 T11153, T12011 T11113, T12011 T11126, T12011 T11132, T12011 T11153, T11805 T4395, T11805 T10998, T11317 T6275, and T11317 T9149, cogcog B T11997 T4395, T11997 T11110, T11997 T11113, T11997 T11125, T11997 T11132, T11997 T11153, and T11801 T10998, cogcog heterozygotes C T11805 T11089, T11317 T11045, T11281 T11311, and T11281 T11313, cog homozygotes D T11801 T11089, T9144 T11311, T9144 T11313, and T9144 T11045, and rec2 heterozygote E T11805 T10997.FIGURE 1. Allelic recombination in diploids heteroallelic for K1201K874.
1998 in regions termed hotspots HOLLIDAY1968. 1992YIP et al. K26K874 diploids yield low frequency of His progeny yielded by each cross, multiplied by In the presenceof rec2, recombination between his3 alleles is reduced 30foldin crosses containing cog and 4fold in crosses of homozygouscog to the same low level ANGEL et al. The centromere, to the left of the figure, is represented by solid disc.
The mutant sites are separated by bp YEADON and CATCHESIDE 1998. The dilution factor between selective and nonselectiveplates varied from 11600 to 1100 for intergenic assays, dependingon the recombination frequency. Statistical analysis of recombination data For his3 K1201K874 heterozygotes Figure 1, his3 K26K874heterozygotes Figure 6, lys4 ad3 Figure 4, and his3 ad3transheterozygotes Figure 5, data from crosses homozygousfor cog were compared to those from crosses heterozygous forcogcog. Therefore,a recombination event initiated at cog YEADON et al. To determine heterogeneity of recombinationfrequencies from repeats of single cross or within singlegenotype, test was used to assess the probability that thecolony counts could differ by chance.
















